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ERIC Number: ED526001
Record Type: Non-Journal
Publication Date: 2009
Pages: 83
Abstractor: As Provided
ISBN: ISBN-978-1-1095-8099-0
Analysis and Characterization of Vitamin B Biosynthesis Pathways in the Phytoparasitic Nematode Heterodera Glycines
Craig, James P.
ProQuest LLC, Ph.D. Dissertation, University of Illinois at Urbana-Champaign
The soybean cyst nematode (SCN), "Heterodera glycines" is an obligate plant parasite that can cause devastating crop losses. To aide in the study of this pathogen, the SCN genome and the transcriptome of second stage juveniles and eggs were shotgun sequenced. A bioinformatic screen of the data revealed nine genes involved in the "de novo" biosynthesis and salvage of the B vitamins: B[subscript 1], B[subscript 5], B[subscript 6], and B[subscript 7]. Each of these "de novo" pathways are believed to have been lost in most animals and their similarity to bacterial homologues made them candidates for horizontal gene transfer (HGT). Each gene had eukaryotic-like introns and poly-A signals confirming that they came from a eukaryote. Four genes had nematode-specific splice leaders attached to their cDNA which confirmed that they came from nematodes. Quantitative polymerase chain reaction on individual nematodes showed similar amplification between the vitamin B biosynthesis genes and other known nematode genes, this further confirmed their presence in the nematode genome. The similarity to bacterial homologues on the amino acid and nucleotide level suggested prokaryotic ancestry and phylogenetic analysis of the genes supported a likely HGT event from bacteria to SCN. HGT was further supported by gene synteny of the two genes "HgSNZ" and "HgSNO." These two genes also were shown to form a complete, functional pathway for vitamin B[subscript 6] biosynthesis, representing the first example the HGT of a complete functional pathway from bacteria into nematodes. The other pathways appear to be missing the initial enzymes for complete de novo biosynthesis of the vitamins, suggesting that the initial substrates are provided by the host. These partial biosynthesis pathways have been found in other single-celled parasites and rhizobia indicating an important role in parasitism and survival within the plant environment. [The dissertation citations contained here are published with the permission of ProQuest LLC. Further reproduction is prohibited without permission. Copies of dissertations may be obtained by Telephone (800) 1-800-521-0600. Web page:]
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Publication Type: Dissertations/Theses - Doctoral Dissertations
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A