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ERIC Number: EJ759173
Record Type: Journal
Publication Date: 2006-Sep
Pages: 9
Abstractor: Author
ISSN: ISSN-1470-8175
Glycobiology, How to Sugar-Coat an Undergraduate Advanced Biochemistry Laboratory
McReynolds, Katherine D.
Biochemistry and Molecular Biology Education, v34 n5 p369-377 Sep 2006
A second semester biochemistry laboratory has been implemented as an independent projects course at California State University, Sacramento since 1999. To incorporate aspects of carbohydrate biochemistry, or glycobiology, into our curriculum, projects in lectin isolation and purification were undertaken over the course of two semesters. Through this modification in course content, this class now offers a diverse, hands-on treatment of not only standard protein purification techniques but also carbohydrate techniques, specifically the study of carbohydrate-protein interactions through hemagglutination assays, a novel commercial assay known as the Instant[TM]Chek assay, and the generation and use of appropriate affinity chromatography matrices. Throughout the semester, the students are in charge of all aspects of their projects, from planning to execution and completion. Specific examples of student projects are highlighted such that the breadth of protein-carbohydrate chemistry pursued in a 15-week semester can be appreciated. The feedback of the course was very favorable, indicating that the students came away with skills necessary for them to be successful in their future careers. Most importantly, however, aspects of glycobiology have now been incorporated effectively into a mainstream undergraduate biochemistry laboratory class.
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Publication Type: Guides - Classroom - Teacher; Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: California