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ERIC Number: EJ985198
Record Type: Journal
Publication Date: 2012-Mar
Pages: 4
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
Electrophoresis Gel Quantification with a Flatbed Scanner and Versatile Lighting from a Screen Scavenged from a Liquid Crystal Display (LCD) Monitor
Yeung, Brendan; Ng, Tuck Wah; Tan, Han Yen; Liew, Oi Wah
Journal of Chemical Education, v89 n4 p513-516 Mar 2012
The use of different types of stains in the quantification of proteins separated on gels using electrophoresis offers the capability of deriving good outcomes in terms of linear dynamic range, sensitivity, and compatibility with specific proteins. An inexpensive, simple, and versatile lighting system based on liquid crystal display backlighting is described. It is used with a flatbed scanner technique previously reported. The results from experimentation and analysis were benchmarked against a commercial densitometer to verify the performance of this analysis system. The availability of this device is expected to be useful in laboratories in schools and in resource-limited venues. (Contains 4 figures.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Evaluative
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A