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Showing 16 to 30 of 207 results Save | Export
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Saperas, Nuria; Fonfria-Subiros, Elsa – Journal of Chemical Education, 2011
This laboratory exercise uses a problem-based approach to expose students to some basic concepts relating to proteins and enzymes. One of the main applications of enzymes at the industrial level is their use in the detergent market. The students examine a detergent sample to ascertain whether proteolytic enzymes are a component and, if so, which…
Descriptors: Evidence, Biochemistry, Science Activities, Scientific Concepts
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Mulvihill, Charlotte – Science Teacher, 2007
At Oklahoma City Community College, they have developed gel electrophoresis activities that support active learning of many scientific concepts, including: pH, electrolysis, oxidation reduction, electrical currents, potentials, conductivity, molarity, gel electrophoresis, DNA and protein separation, and DNA fingerprinting. This article presents…
Descriptors: Scientific Concepts, Active Learning, Genetics, College Science
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Kim, Thomas D.; Craig, Paul A. – Biochemistry and Molecular Biology Education, 2010
Two-dimensional gel electrophoresis (2DGE) remains an important tool in the study of biological systems by proteomics. While the use of 2DGE is commonplace in research publications, there are few instructional laboratories that address the use of 2DGE for analyzing complex protein samples. One reason for this lack is the fact that the preparation…
Descriptors: Undergraduate Study, Molecular Biology, Biochemistry, Laboratories
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Yeung, Brendan; Ng, Tuck Wah; Tan, Han Yen; Liew, Oi Wah – Journal of Chemical Education, 2012
The use of different types of stains in the quantification of proteins separated on gels using electrophoresis offers the capability of deriving good outcomes in terms of linear dynamic range, sensitivity, and compatibility with specific proteins. An inexpensive, simple, and versatile lighting system based on liquid crystal display backlighting is…
Descriptors: Science Laboratories, Lighting, Chemistry, College Science
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Phillips, Allison R.; Robertson, Amber L.; Batzli, Janet; Harris, Michelle; Miller, Sarah – CBE - Life Sciences Education, 2008
Polymerase chain reaction (PCR) and gel electrophoresis have become common techniques used in undergraduate molecular and cell biology labs. Although students enjoy learning these techniques, they often cannot fully comprehend and analyze the outcomes of their experiments because of a disconnect between concepts taught in lecture and experiments…
Descriptors: Cytology, Biology, Misconceptions, Teaching Methods
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Hijnen, Hens – Journal of Chemical Education, 2009
A theoretical description of the influence of electroosmosis on the effective mobility of simple ions in capillary zone electrophoresis is presented. The mathematical equations derived from the space-charge model contain the pK[subscript a] value and the density of the weak acid surface groups as parameters characterizing the capillary. It is…
Descriptors: Prediction, Equations (Mathematics), Science Instruction, Chemistry
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Mills, Nancy S.; Spence, John D.; Bushey, Michelle M. – Journal of Chemical Education, 2005
A series of substituted benzoic acids (SBAs) are prepared by students. The pKa shift, a result of the electron-withdrawing or electron-donating characteristics of the subsistent is examined in reference to the electrophoretic migration behavior of benzoic acid.
Descriptors: Chemistry, Science Education, Science Experiments, Stoichiometry
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Anderson, Greg; Thompson, Jonathan E.; Shurrush, Khriesto – Journal of Chemical Education, 2006
We describe an inexpensive device for performing capillary electrophoresis (CE) separations with fluorescence detection. As a demonstration of the device's utility we have determined the mass of riboflavin in a commercially available dietary supplement. The device allows for separation of riboflavin in [asymptotically equivalent to] 100 s with a…
Descriptors: Dietetics, Nutrition, Science Instruction, Chemistry
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Maderia, Vitor M. C.; Pires, Euclides M. V. – Journal of Chemical Education, 1986
Discusses the value of electrophoresis in the fields of protein chemistry and biochemistry. Describes how to build an inexpensive electrophoresis setup for use in either research or teaching activities. Details the construction of both the separating device and the power supply. (TW)
Descriptors: Building Plans, Chemical Analysis, Chemistry, College Science
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Wilkesman, Jeff; Castro, Diana; Contreras, Lellys M.; Kurz, Liliana – Biochemistry and Molecular Biology Education, 2014
This laboratory exercise presents a novel way to introduce undergraduate students to the specific detection of enzymatic activity by electrophoresis. First, students prepare a crude peroxidase extract and then analyze the homogenate via electrophoresis. Zymography, that is, a SDS-PAGE method to detect enzyme activity, is used to specifically…
Descriptors: Science Laboratories, College Science, Undergraduate Students, Molecular Structure
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Moertel, Cheryl; Frutiger, Bruce – Science Teacher, 1996
Describes a DNA fingerprinting simulation that uses vegetable food coloring and plastic food containers instead of DNA and expensive gel electrophoresis chambers. Allows students to decipher unknown combinations of dyes in a method similar to that used to decipher samples of DNA in DNA fingerprint techniques. (JRH)
Descriptors: Chemical Analysis, DNA, Genetics, Science Activities
Brewer, John M.; Ashworth, Raymond B. – J Chem Educ, 1969
Descriptors: College Science, Electricity, Laboratory Equipment, Laboratory Procedures
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Szeberényi, József – Biochemistry and Molecular Biology Education, 2014
Terms to be familiar with before you start to solve the test: Southern blotting, Western blotting, restriction endonucleases, agarose gel electrophoresis, nitrocellulose filter, molecular hybridization, polyacrylamide gel electrophoresis, proto-oncogene, c-abl, Src-homology domains, tyrosine protein kinase, nuclear localization signal, cDNA,…
Descriptors: Problem Solving, Molecular Biology, Genetics, Tests
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Costa, M. J. – Biochemistry and Molecular Biology Education, 2007
Undergraduates with little training on molecular biology may find the technical level of the typical introductory restriction laboratory too challenging and have problems with mastering the underlying concepts and processes. "Blackboard electrophoresis" is an active learning exercise, which focuses student attention on the sequences and principles…
Descriptors: Teaching Methods, Laboratory Equipment, Chalkboards, Molecular Biology
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Erasmus, Daniel J. – Biochemistry and Molecular Biology Education, 2021
Education in biochemistry teaching laboratories focus primarily on applying biochemical techniques to understanding human disease, biochemistry, and biotechnology. With anthropogenic climate change, there is a renewed interest in quantifying biodiversity, especially with the use of molecular-based approaches such as DNA barcoding. This 3-week…
Descriptors: Biochemistry, College Science, Undergraduate Students, Science Laboratories
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