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ERIC Number: EJ951468
Record Type: Journal
Publication Date: 2011-Nov
Pages: 2
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
Isolation of Cholesterol from an Egg Yolk
Taber, Douglass F.; Li, Rui; Anson, Cory M.
Journal of Chemical Education, v88 n11 p1580-1581 Nov 2011
A simple procedure for the isolation of the cholesterol, by hydrolysis and extraction followed by column chromatography, is described. The cholesterol can be further purified by complexation with oxalic acid. It can also be oxidized and conjugated to cholestenone. The source of the cholesterol is one egg yolk, which contains about 200 mg of lipid-bound cholesterol. This experiment is appropriate for an undergraduate organic chemistry laboratory and can be completed in 3 h. (Contains 1 figure.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A