ERIC Number: EJ1100257
Record Type: Journal
Publication Date: 2016
Abstractor: As Provided
An Undergraduate Laboratory Class Using CRISPR/Cas9 Technology to Mutate Drosophila Genes
Adame, Vanesa; Chapapas, Holly; Cisneros, Marilyn; Deaton, Carol; Deichmann, Sophia; Gadek, Chauncey; Lovato, TyAnna L.; Chechenova, Maria B.; Guerin, Paul; Cripps, Richard M.
Biochemistry and Molecular Biology Education, v44 n3 p263-275 May-Jun 2016
CRISPR/Cas9 genome editing technology is used in the manipulation of genome sequences and gene expression. Because of the ease and rapidity with which genes can be mutated using CRISPR/Cas9, we sought to determine if a single-semester undergraduate class could be successfully taught, wherein students isolate mutants for specific genes using CRISPR/Cas9. Six students were each assigned a single Drosophila gene, for which no mutants currently exist. Each student designed and created plasmids to encode single guide RNAs that target their selected gene; injected the plasmids into "Cas9"-expressing embryos, in order to delete the selected gene; carried out a three-generation cross to test for germline transmission of a mutated allele and generate a stable stock of the mutant; and characterized the mutant alleles by PCR and sequencing. Three genes out of six were successfully mutated. Pre- and post-survey evaluations of the students in the class revealed that student attitudes towards their research competencies increased, although the changes were not statistically significant. We conclude that it is feasible to develop a laboratory genome editing class, to provide effective laboratory training to undergraduate students, and to generate mutant lines for use by the broader scientific community.
Descriptors: Undergraduate Students, Laboratory Experiments, Genetics, Student Surveys, Student Attitudes, Science Instruction, Teaching Methods, Entomology, Technology Uses in Education
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Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education; Postsecondary Education
Sponsor: National Institute of General Medical Sciences (NIH//DHHS)
Authoring Institution: N/A