Today, use of the Web 2.0 technologies and applications (e.g. text messaging, wikis, personal web pages, social networks, blogs) in many different areas of education have been increasing, and this can be seen as an important development. However, teaching candidates from specific subject matters, such as physical education, still appear unaware of the potential benefits of such tools in a teaching and learning environment. The purpose of this study was to assess pre-service teachers' perceptions about Web 2.0 technologies and to explore the awareness of this technology among students using the Technology Acceptance Modal (TAM). In this context, the descriptive survey method was used and a questionnaire was applied to 79 pre-service students (53 male and 26 female) enrolled in the physical education department in Dokuz Eylul University. The data for this study was collected by the researcher through a questionnaire after 2017-2018 fall semester of the course including weekly web 2.0 activities based on the lesson topic. The results of the study indicate that gender was an important factor that affected the implementation or use of this technology. Accordingly, male students had higher levels of awareness about web 2.0 applications than female students had. The study also showed that usefulness and ease-of-use are two significant factors that affect students' attitude towards this technology.