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ERIC Number: EJ1269268
Record Type: Journal
Publication Date: 2020-Aug
Pages: 4
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: N/A
Modied Method for Extraction of Photosynthetic Plant Pigments for Microcolumn Chromatography
Ciaccio, James A.; Hassan, Kareem
Journal of Chemical Education, v97 n8 p2362-2365 Aug 2020
Column chromatography of photosynthetic plant pigments is a common experiment in introductory laboratory courses for science majors and nonscience majors alike. We report a simple, rapid, reproducible, and small-scale solid--liquid extraction of photosynthetic pigments from inexpensive, store-bought dried herbs that affords a dark-green extract solution, all of which can be applied directly to a pipet microcolumn made from as little as 0.75 g of silica gel. Carotenes and chlorophylls are cleanly separated using standard steps in microcolumn chromatography: the former elute together within 20 min using gravity and 9:1 (v/v) heptane:EtOAc, and the latter elute together within seconds using air pressure and EtOAc.
Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A