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The Real Reason Why ATP Hydrolysis Is Spontaneous at pH > 7: It's (Mostly) the Proton Concentration!
Peer reviewed
Direct linkSilverstein, Todd P. – Biochemistry and Molecular Biology Education, 2023
Common wisdom holds that ATP hydrolysis is spontaneous because of the weakness of its phosphoanhydride bonds, electrostatic repulsion within the polyanionic ATP[superscript 4]-molecule, and resonance stabilization of the inorganic phosphate and ADP products. By examining the pH-dependence of the hydrolysis Gibbs free energy, we show that in fact,…
Descriptors: Biochemistry, Molecular Biology, Science Education, Scientific Concepts
Download full textRoberto Reinoso-Tapia; Sara Galindo; Jaime Delgado-Iglesias; Javier Bobo-Pinilla – Journal of Turkish Science Education, 2024
The aim of this research was to evaluate and compare the efficiency of the flipped learning strategy with that of a conventional teaching method with respect to learning outcomes, cognitive gain, and perception and satisfaction with regard to the methodology used. The research was carried out during the 2021-2022 academic year and focused on a…
Descriptors: Blended Learning, Molecular Biology, Preservice Teachers, Academic Achievement
Naoto Shirasu; Shin'ichiro Yasunaga – Biochemistry and Molecular Biology Education, 2024
Here, we propose a laboratory exercise to quickly determine single nucleotide polymorphisms (SNPs) in human "alcohol dehydrogenase 1B (ADH1B)" and "aldehyde dehydrogenase 2 (ALDH2)" genes involved in alcohol metabolism. In this exercise, two different genotyping methods based on polymerase chain reaction (PCR), namely…
Descriptors: Genetics, Biochemistry, Students, Science Instruction
Tiantian Cheng; Lingzhen Cao; Meng Zou – American Biology Teacher, 2024
The central dogma of molecular biology describes the transfer of genetic information from nucleic acids to proteins and stipulates that the system cannot work in the reverse direction. As a fundamental principle in biology, the dogma is as influential as it is controversial. Some commentators have debated the central dogma's empirical accuracy…
Descriptors: Biology, Genetics, Science Instruction, Physiology
D'Onofrio, María Guillermina; Rogers, Juan D. – Research Evaluation, 2022
The evaluation system is an important component of the institutional arrangements that may shape the career trajectories of researchers. Using logistic regression and recursive partition models, we analyze the resulting key individual level factors that seem to play an important role in the promotion of researchers through the research evaluation…
Descriptors: Research, Researchers, Foreign Countries, Molecular Biology
Craig, Paul A.; Nash, Jessica A.; Crawford, T. Daniel – Biochemistry and Molecular Biology Education, 2022
A programming workshop has been developed for biochemists and molecular biologists to introduce them to the power and flexibility of solving problems with Python. The workshop is designed to move users beyond a "plug-and-play" approach that is based on spreadsheets and web applications in their teaching and research to writing scripts to…
Descriptors: Programming Languages, Biochemistry, Molecular Biology, Data Analysis
Asmus, Stephen E.; Wells, Collin K.; Montalvo, Hanna M. – Biochemistry and Molecular Biology Education, 2023
Heart muscle cells, or cardiomyocytes, exhibit intrinsic contractility in vitro. We found that commercially-available mammalian cardiomyocytes serve as an excellent model system for studying the cytoskeleton and cellular contractility, fundamental topics in undergraduate cell and molecular biology courses. Embryonic rat cardiomyocytes were plated…
Descriptors: Anatomy, Cytology, Science Laboratories, Laboratory Equipment
Josep J. Centelles; Estefania Moreno; Pedro R. de Atauri – International Society for Technology, Education, and Science, 2023
Gamification is a very useful technique, that has been fully increasing in the last years. During the COVID-19 pandemic, our innovation teaching group sent to our students several games, that were used by them for self-studying. Considering that many numerical problems can be proposed in Biochemistry, rather than numerical games (sudoku, adding…
Descriptors: Gamification, Biochemistry, Vocabulary, Educational Games
Ludmila Tyler; Peter J. Kennelly; Shelly Engelman; Kirsten F. Block; Jennifer C. Bobenko; Jaclyn Catalano; Jesica A. Jones; Margaret I. Kanipes-Spinks; Yang Mooi Lim; Jennifer Loertscher; Tejiri Olafimihan; Hailey Reiss; Territa L. Upchurch-Poole; Yufeng Wei; Kimberly J. Linenberger Cortes; Victoria Del Gaizo Moore; Daniel R. Dries – Biochemistry and Molecular Biology Education, 2024
We present as a case study the evolution of a series of participant-centered workshops designed to meet a need in the life sciences education community--the incorporation of best practices in the assessment of student learning. Initially, the ICABL (Inclusive Community for the Assessment of Biochemistry and Molecular Biology/BMB Learning) project…
Descriptors: Science Education, Science Teachers, Teacher Workshops, Biological Sciences
Martina Blecic – Journal of Biological Education, 2024
In this paper I suggest a pragmatic model for the notion of 'information' used in molecular biology in the description of protein synthesis. Discarding any ontological commitments of the term 'information', I propose a view of information based on an analogy with communication. This view could at least supplement the existing information-metaphor…
Descriptors: Genetics, Molecular Biology, Figurative Language, Communication (Thought Transfer)
Shane Terrell Jinson Jr. – ProQuest LLC, 2024
Understanding the details of how scientific breakthroughs and discoveries emerge from prior work, knowledge, and collaboration is fundamental to science history. Traditional methods of collecting such relevant information and transforming it into rich narratives have existed since the inception of the scientific method. These typically involve…
Descriptors: Biological Sciences, Qualitative Research, Cytology, Authors
Chang, Ming-Mei – Biochemistry and Molecular Biology Education, 2022
Southern blot analysis is an important molecular biology technique for identifying a specific sequence in DNA samples. Although it is no longer used extensively in recent years, the steps and underlying principles of Southern blot are applicable to modern biology. High sensitivity and limited background are keys to successful Southern blots,…
Descriptors: Molecular Biology, Science Instruction, Genetics, Hands on Science
Singh, Anoushka; Miller, Ryan C.; Archuleta, Stephen R.; Kugel, Jennifer F. – Biochemistry and Molecular Biology Education, 2023
Transcription is the critical first step in expressing a gene, during which an RNA polymerase (RNAP) synthesizes an RNA copy of one strand of the DNA that encodes a gene. Here we describe a laboratory experiment that uses a single assay to probe two important steps in transcription: (1) RNAP binding to DNA, and (2) the transcriptional activity of…
Descriptors: Molecular Biology, Science Instruction, Genetics, Teaching Methods
Leonie I. Johann; Fredrik K. H. Rusk; Michael J. Reiss; Jorge Groß – Journal of Biological Education, 2024
This study reports on the theoretical- and empirical-based design and evaluation of cell membrane biology learning activities within the Model of Educational Reconstruction and experiential realism. First, we designed analogy-based learning activities by considering students' and scientists' conceptions as described in the literature. Secondly, we…
Descriptors: Secondary School Students, Thinking Skills, Cytology, Molecular Biology
Risanti Dhaniaputri; Hadi Suwono; Betty Lukiati – Journal of Biological Education Indonesia (Jurnal Pendidikan Biologi Indonesia), 2024
Plant metabolite compounds have been applied on plant cellular metabolism, produces organic and inorganic compounds, primary and secondary bioactive molecules, such as glucose, amino acids, fat acids, alkaloids, flavonoids, and terpenoids. Undergraduate biology students learn about the plant terpenoid assisted by bioinformatics to store, manage,…
Descriptors: Foreign Countries, Science Instruction, Biology, Plants (Botany)
