The present study aimes to identify the reasons behind experiencing job alienation by the faculty members at Hebron & Al-Quds universities. It was conducted during the second semester of the academic year (2017/2018). The study's population involves all the faculty members who work at Hebron & Al-Quds universities (i.e. 446 faculty members). The study's sample consists of (200) faculty members. Those members were selected through using the random stratified sampling method. The study's instrument was developed by the researchers, and It is represented in a questionnaire. The questionnaire seeks to identify the reasons behind experiencing job alienation by the faculty members at Hebron & Al-Quds universities. It consists of (20) statements. These statements address two types of reasons; a)- reasons that are related to the university management & b)- reasons that are related to the work colleagues. The researchers checked the validity of questionnaire, and they measured the reliability of the questionnaire through using the relevant statistical methods. It was found that the statements that concern the examined reasons show moderate means. In addition, it was found that there is not any statistically significant difference between the respondents' attitudes which can be attributed to the university.