The evolution of learning design continues, focusing on blended laboratory approaches incorporating technology. The Sophisticated Thinking Laboratory (STB-LAB) and Gather Town are key tools in implementing collaborative learning in this context. This study assesses the effectiveness of blended laboratory implementation using STB-LAB and Gather Town as a gamification tool, utilizing the Assessment Based on Teaching and Learning Trajectory (AABTLT) with Student Activity Sheets (SAS). The results reveal the successful execution of STB-LAB syntax in blended laboratory activities. Additionally, STB-LAB and Gather Town significantly enhance students' collaborative skills, as indicated by a substantial Cohen's D Effect Size. For physics education majors, the effect size is 1.736 in the experiment group and 0.754 in the control group, while for biology education majors, it is 1.522 in the experiment group and 0.541 in the control group. This study highlights the positive impact of blended laboratories with STB-LAB and Gather Town on collaborative skill development, further emphasizing the role of technology in contemporary learning design. [Note: The page range (66-77) shown on the PDF citation is incorrect. The correct page range is 67-78.]