This study investigates the change blindness that may occur in multimedia learning environments. For this purpose, a multimedia animation which had some changes was designed. The eye movements were examined during the process of detecting the changes in multimedia via eye tracking technics. The research model was defined as a controlled experiment method. Fifteen ungraduated students participated in the experiment. Attention levels of participants were determined by d2 Attention Test. Change detection numbers of participants were analyzed according to their attention level and their gender. The appearance of a major object on the scene was the most detected change and the change on the detail object was detected less. According to findings about the attention level and change detection, the participants at high attention level were more successful at detecting change in multimedia. Females were more successful in detecting change than males.