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ERIC Number: EJ923708
Record Type: Journal
Publication Date: 2011-Feb
Pages: 4
Abstractor: As Provided
ISSN: ISSN-0021-9584
Exploring Liquid Sequential Injection Chromatography to Teach Fundamentals of Separation Methods: A Very Fast Analytical Chemistry Experiment
Penteado, Jose C.; Masini, Jorge Cesar
Journal of Chemical Education, v88 n2 p235-238 Feb 2011
Influence of the solvent strength determined by the addition of a mobile-phase organic modifier and pH on chromatographic separation of sorbic acid and vanillin has been investigated by the relatively new technique, liquid sequential injection chromatography (SIC). This technique uses reversed-phase monolithic stationary phase to execute fast separations (less than 1 min), allowing the students to quickly investigate the effect of analytes distribution (protonated and unprotonated) on the separation of compounds having acid-base properties. Design of experiments (DOE) was used to find the suitable condition for optimal separation in short chromatographic runs. The experiment is suitable for any chemistry student who has completed a minimum of one year of general chemistry, having a solid background in fundamentals of analytical chemistry. It is appropriate for an instrumental analysis course either for upper-division undergraduate or graduate students. (Contains 1 table and 2 figures.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A