ERIC Number: EJ1084278
Record Type: Journal
Publication Date: 2015-Dec
Abstractor: As Provided
A Size Exclusion Chromatography Laboratory with Unknowns for Introductory Students
McIntee, Edward J.; Graham, Kate J.; Colosky, Edward C.; Jakubowski, Henry V.
Journal of Chemical Education, v92 n12 p2126-2129 Dec 2015
Size exclusion chromatography is an important technique in the separation of biological and polymeric samples by molecular weight. While a number of laboratory experiments have been published that use this technique for the purification of large molecules, this is the first report of an experiment that focuses on purifying an unknown small molecule from a protein. Students must then determine the structure of the unknown using spectral determination.
Descriptors: Science Instruction, College Science, Undergraduate Study, Chemistry, Science Laboratories, Science Experiments, Laboratory Experiments, Molecular Structure, Scientific Concepts
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: firstname.lastname@example.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Sponsor: National Science Foundation (NSF)
Authoring Institution: N/A
Grant or Contract Numbers: 1043566