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ERIC Number: EJ1036377
Record Type: Journal
Publication Date: 2014-Aug
Pages: 4
Abstractor: As Provided
ISSN: ISSN-0021-9584
Using Ion Exchange Chromatography to Separate and Quantify Complex Ions
Johnson, Brian J.
Journal of Chemical Education, v91 n8 p1212-1215 Aug 2014
Ion exchange chromatography is an important technique in the separation of charged species, particularly in biological, inorganic, and environmental samples. In this experiment, students are supplied with a mixture of two substitution-inert complex ions. They separate the complexes by ion exchange chromatography using a "flash" technique. After taking a visible spectrum of the eluted complexes, the unknowns are identified by comparison with a table of supplied ?[subscript max] values and quantified using Beer's law. This experiment can be completed in under 4 h and is suitable for introductory and intermediate chemistry students.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: National Science Foundation
Authoring Institution: N/A
Grant or Contract Numbers: NSF-DUE 1043566