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ERIC Number: EJ1036381
Record Type: Journal
Publication Date: 2014-Aug
Pages: 4
Abstractor: As Provided
ISSN: ISSN-0021-9584
Integrating Biology into the General Chemistry Laboratory: Fluorometric Analysis of Chlorophyll "a"
Wesolowski, Meredith C.
Journal of Chemical Education, v91 n8 p1224-1227 Aug 2014
A laboratory experiment that introduces fluorometry of chlorophyll "a" at the general chemistry level is described. The use of thin-layer chromatography to isolate chlorophyll "a" from spirulina and leaf matter enables quantification of small amounts of chlorophyll "a" via fluorometry. Student results were reasonably comparable to those found in the literature. This experiment uses relatively low volumes of organic solvents and an inexpensive fluorescence spectrometer (such as a Vernier SpectroVis Plus). The method could also be adapted for inquiry-based laboratories comparing chlorophyll a levels in plants, algae, and photosynthetic bacteria.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A