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Hancock, Dale; Funnell, Alister; Jack, Briony; Johnston, Jill – Biochemistry and Molecular Biology Education, 2010
An experiment is conducted, which in four 3 h laboratory sessions, introduces third year undergraduate Biochemistry students to the technique of real-time PCR in a biological context. The model used is a murine erythroleukemia cell line (MEL cells). These continuously cycling, immature red blood cells, arrested at an early stage in erythropoiesis,…
Descriptors: Undergraduate Students, Genetics, Cytology, Biochemistry
Hancock, Dale; Nguyen, Lisa L.; Denyer, Gareth S.; Johnston, Jill M. – Biochemistry and Molecular Biology Education, 2006
A microarray experiment is presented that, in six laboratory sessions, takes undergraduate students from the tissue sample right through to data analysis. The model chosen, the murine erythroleukemia cell line, can be easily cultured in sufficient quantities for class use. Large changes in gene expression can be induced in these cells by…
Descriptors: Undergraduate Students, Laboratories, Data Analysis, Quality Control