ERIC Number: EJ1119573
Record Type: Journal
Publication Date: 2016-Nov
Abstractor: As Provided
Determination of Total Arsenic and Speciation in Apple Juice by Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry: An Experiment for the Analytical Chemistry Laboratory
He, Ping; Colon, Luis A.; Aga, Diana S.
Journal of Chemical Education, v93 n11 p1939-1944 Nov 2016
A two-part laboratory experiment was designed for upper-level analytical chemistry students to provide hands-on experience in the use of high performance liquid chromatography (HPLC) for separation and inductively coupled plasma mass spectrometry (ICP-MS) for detection. In the first part of the experiment, the students analyze total arsenic in apple juice purchased from local grocery stores using direct injection ICP-MS. In the second part, different species of arsenic in the same apple juice samples are determined using HPLC--ICP-MS. Quantification is performed based on two methods, standard addition and external calibration curve, to demonstrate how sample matrix can affect the accuracy of the analysis. The experiments provide the opportunity to introduce and/or review several fundamental analytical chemistry concepts: chromatographic separations, mass spectrometry, inductively couple plasma, matrix effects, calibration methods, internal standard, and basic statistical tests. Undergraduate students enrolled in the Instrumental Analysis Laboratory class at the University at Buffalo performed this experiment successfully during the 2015 spring semester.
Descriptors: Science Instruction, Science Experiments, Laboratory Experiments, Science Laboratories, Hands on Science, Chemistry, College Science, Spectroscopy, Undergraduate Students, Food
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: firstname.lastname@example.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Sponsor: National Science Foundation (NSF)
Authoring Institution: N/A
Identifiers - Location: New York (Buffalo)
Grant or Contract Numbers: CHE0959565; CHE1508105