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ERIC Number: EJ1166995
Record Type: Journal
Publication Date: 2018-Jan
Pages: 6
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
Biological Interaction of Molybdenocene Dichloride with Bovine Serum Albumin Using Fluorescence Spectroscopy
Domínguez, Moralba; Cortes-Figueroa, Jose´ E.; Meléndez, Enrique
Journal of Chemical Education, v95 n1 p152--157 Jan 2018
Bioinorganic topics are ubiquitous in the inorganic chemistry curriculum; however, experiments to enhance understanding of related topics are scarce. In this proposed laboratory, upper undergraduate students assess the biological interaction of molybdenocene dichloride (Cp2MoCl2) with bovine serum albumin (BSA) by fluorescence spectroscopy. Specifically, learners study the quenching mechanism by performing a binding titration of a bovine serum albumin (BSA) solution with molybdenocene dichloride at physiological pH at three temperatures to determine the biomolecular quenching constant as defined in the Stern-Volmer equation. The temperature dependency of the quenching constant allows estimation of thermodynamic parameters which in turn permits an assessment of the nature of the intermolecular interactions involved. This educational activity promotes graph interpretation and integration of concepts such as metallocene-protein interaction, fluorescence quenching, Gibbs energy, entropy, and enthalpy, where students learn to propose a quenching mechanism and to assess the intermolecular forces that may be involved. The proposed experiment can be implemented in various educational settings such as inorganic chemistry, biochemistry, biophysical chemistry, and analytical chemistry.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: Puerto Rico