ERIC Number: EJ1012182
Record Type: Journal
Publication Date: 2013-Feb
Pages: 7
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: N/A
Surface Plasmon Resonance Spectroscopy: A Versatile Technique in a
Biochemist's Toolbox
Bakhtiar, Ray
Journal of Chemical Education, v90 n2 p203-209 Feb 2013
Surface plasmon resonance (SPR) spectroscopy is a powerful, label-free technique to monitor noncovalent molecular interactions in real time and in a noninvasive fashion. As a label-free assay, SPR does not require tags, dyes, or specialized reagents (e.g., enzymes-substrate complexes) to elicit a visible or a fluorescence signal. During the last two decades, SPR has been broadly applied to study of noncovalent interactions of protein-DNA, protein-cell, RNA-DNA, DNA-DNA, protein-protein, protein-carbohydrate, small molecule-macromolecule (e.g., receptor-inhibitor complex), protein-peptide, and self-assembled monolayers. In addition, SPR has been successfully applied to drug discovery ligand-fishing and clinical immunogenicity studies (i.e., to monitor an immune response against a therapeutic agent). SPR spectroscopy can address questions such as specificity of an interaction, kinetics, affinity, and concentrations of selected molecules present in a sample of interest. Given the current enhancements in hardware and software capabilities along with its ease of use and maintenance, SPR experiments can be designed for upper-level undergraduate biochemistry, biophysics, and physical chemistry laboratory courses. In this article, an overview of SPR phenomenon, instrumentation, sensor immobilization, and its selected applications is presented. (Contains 2 tables and 5 figures.)
Descriptors: Chemistry, Science Instruction, Spectroscopy, Biochemistry, Molecular Biology, College Science, Hands on Science, Scientific Concepts, Light, Computer Software, Educational Technology, Graduate Students, Undergraduate Students, Laboratory Experiments, Laboratory Equipment
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A