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ERIC Number: EJ991017
Record Type: Journal
Publication Date: 2012
Pages: 2
Abstractor: ERIC
ISSN: ISSN-1470-8175
Problem-Solving Test: The Mechanism of Transcription Termination by the Rho Factor
Szeberenyi, Jozsef
Biochemistry and Molecular Biology Education, v40 n2 p140-141 Mar-Apr 2012
Transcription termination comes in two forms in "E. coli" cells. Rho-dependent termination requires the binding of a termination protein called Rho factor to the transcriptional machinery at the terminator region, whereas Rho-independent termination is achieved by conformational changes in the transcript itself. This article presents a test experiment that was designed to study the mechanism of action of the Rho protein. Artificial substrates to analyze Rho action were constructed and tested in cell-free reactions. A recombinant plasmid was prepared by inserting a short sequence into the plasmid DNA. (Contains 1 figure and 1 footnote.)
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Publication Type: Journal Articles; Reports - Evaluative
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A