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Direct linkPrimo, Emiliano D.; Cossovich, Sacha; Giordano, Walter – Journal of Biological Education, 2020
"Sinorhizobium meliloti" is a bacterium of great agroeconomic importance because of its ability to fix atmospheric nitrogen in symbiotic association with alfalfa ("Medicago sativa") roots, and it is often used in model studies. We investigated the effects of exopolysaccharides (EPSs) in cell-to-cell and cell-to-surface…
Descriptors: Science Instruction, Undergraduate Students, College Science, Science Laboratories
Werby, Sabrina H.; Cegelski, Lynette – Journal of Chemical Education, 2019
A six-laboratory session biofilm-focused biochemistry laboratory module is described. Through just six four-hour laboratory sessions over three weeks, students first master background knowledge and fundamental experimental techniques to explore questions involving bacterial biofilms at the interface of microbiology and biochemistry and then…
Descriptors: Laboratory Experiments, Science Experiments, Undergraduate Students, Design
Download full textCandal, Carmen – NCSSSMST Journal, 2012
The effectiveness of five different enzymes as treatments against Staphylococcus biofilm growth was measured in the presence of antibiotics and alone. Protease was the least effective enzyme in biofilm removal with all antibiotics, and pectinase was the most effective with dicloxacillin and clindamycin. Also, dicloxacillin was the most effective…
Descriptors: Experimental Groups, Comparative Analysis, Control Groups, Biochemistry
Mussi, María Alejandra; Actis, Luis A.; de Mendoza, Diego; Cybulski, Larisa E. – Biochemistry and Molecular Biology Education, 2014
A laboratory exercise was designed to illustrate how physical stimuli such as temperature and light are sensed and processed by bacteria to elaborate adaptive responses. In particular, we use the well-characterized Des pathway of "Bacillus subtilis" to show that temperature modulates gene expression, resulting ultimately in modification…
Descriptors: Science Laboratories, Stimuli, Heat, Light
Lee, Kilho; Comolli, Noelle K.; Kelly, William J.; Huang, Zuyi – Chemical Engineering Education, 2015
Mathematical models play an important role in biochemical engineering. For example, the models developed in the field of systems biology have been used to identify drug targets to treat pathogens such as Pseudomonas aeruginosa in biofilms. In addition, competitive binding models for chromatography processes have been developed to predict expanded…
Descriptors: Chemical Engineering, Biochemistry, College Science, Science Instruction
Paulino, Tony P.; Andrade, Ricardo O.; Bruschi-Thedei, Giuliana C. M.; Thedei, Geraldo, Jr.; Ciancaglini, Pietro – Biochemistry and Molecular Biology Education, 2004
The main objective of this class experiment is to show the influence of carbon source and of different fluoride concentrations on the biofilm formation by the bacterium "Streptococcus mutans." The observation of different biofilm morphology as a function of carbon source and fluoride concentration allows an interesting discussion regarding the…
Descriptors: Prevention, Diseases, Biochemistry, Microbiology
