The aim of this study is to investigation the opinions of science high school students about physical education and sports lesson. The research is designed with an exploratory sequential pattern from mixed research approaches. The research was carried out in two Science High Schools in Kirikkale province during the fall semester of 2017-2018 academic years. The study was conducted on three groups [first study group: 245 (141 females; 104 males); second study group: 24 (14 females; 10 males); third study group: 8 (7 female; 1 male)]. Quantitative data were obtained through the "Personal Information Form" prepared by the researcher and the "Physical Education Attitude Scale for Secondary School Students" developed by Güllü and Güçlü (2009). Qualitative data were collected by using the drawing-writing form and semi-structured interview form. Descriptive statistics, independent group t-test, one-way analysis of variance and Pearson correlation analysis were used to analyze the quantitative data. In the analysis of qualitative data, content analysis was utilized. According to the findings, it was found that the attitudes of science high school students towards physical education lesson were at a moderate level and male students had a more positive attitude than female students. There was no difference between academic achievement and class level.