The aim of this study is to define the effects of gender on attitudes of preservice teachers towards the teaching profession in Turkey. It combines the findings of 35 relevant studies comprising a sample of 4,289 male and 6,073 female preservice teachers. A Group Difference model was used to identify fixed and random effects and to facilitate comparison using meta-analysis methods. A significant effect size (d = 0.271) on an insignificant level was identified in favour of the gender attitudes of female preservice teachers. The location of the research, branch, and class level/educational status were also found to have moderating effects on attitudes.