This study examined the attitudes, preparation, and comfort of early childhood administrators in working with gay and lesbian (GL) parented families and the use of GL inclusive practices within centers. Data were gathered from 203 participants in the state of North Carolina using an online survey. Overall, administrators held a positive attitude towards GLs. Specifically, administrators with higher levels of education held a more positive attitude towards lesbians than gay men. Attitudes also correlated highly with administrator's comfort in working with GL parented families and use of inclusive practices within their center; however, it did not correlate with preparation or training in the field. Participants who identified themselves as very religious had lower scores on all measures used within the study, compared to administrators who were somewhat religious or not religious. Finally, most of the inclusive strategies implemented within centers were perfunctory, which required minimal change and effort by administrators.