A considerable number of foreign language learners experience a feeling of anxiety in language learning process. The purpose of this research was to find out foreign language anxiety levels of students studying in the Faculty of English Language and Literature at Cumhuriyet University, Sivas, Turkey when they were in preparatory class and when they were in fourth grade. Furthermore, it was aimed to examine whether gender factor was effective on foreign language anxiety of students. The research was carried out on 98 students (57 female) continuing from preparatory class to fourth grade and taking part in the first and second phases of the study voluntarily. Research data were collected through Foreign Language Classroom Anxiety Scale. Although foreign language anxiety levels of the students were found at moderate level both in preparatory class and in fourth grade, students had slightly higher anxiety level in fourth grade than they had in preparatory class. However, foreign language anxiety among males was found higher than females.