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ERIC Number: EJ1215750
Record Type: Journal
Publication Date: 2019-May
Pages: 6
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
Functionalization of Gold Nanoparticles for a Color-Based Detection of Adenosine in a Bioassay
Roupioz, Yoann
Journal of Chemical Education, v96 n5 p1002-1007 May 2019
A simple bioassay was designed for the detection of adenosine, a human metabolite involved in several physiological processes, on the basis of the functionalization of gold nanoparticles with DNA oligonucleotides selectively binding adenosine. The test was possible thanks to the selective and sensitive binding of adenosine by two DNA oligonucleotides forming an aptamer. Each DNA strand was grafted on the surface of gold nanoparticles, which formed an insoluble aggregate within a few minutes in the presence of millimolar concentrations of adenosine. The experiments described are suitable for undergraduate and graduate students developing skills in handling and functionalizing nanomaterials for use in biosensors. Besides the handling of nanomaterials, this laboratory training enabled the students to familiarize themselves with the use of nucleic acids in novel techniques, for example, as efficient probes for biosensing purposes.
Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Guides - Classroom - Learner; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: Students
Language: English
Sponsor: N/A
Authoring Institution: N/A