Using technology in a science teaching is so important. Only the person, who can use these tools in expert level, can use these tools in their teaching activities. In this research it is aimed firstly identifying science teacher candidates web 2.0 tools usage experience level and factors affecting experience level. In this research survey method was adapted. To gather research data a survey was developed. Survey contains seven sections concerning demographic data, blog, wiki, image sharing, document sharing, social network site and instant messaging usage. After reviewing literature survey developed and survey was given CEIT and science education experts to review. After expert examination survey, finalized survey administrated 289 science teacher candidates and obtained data analyzed.Research findings show that science teacher candidates experience regarding each tool fairly equal and their experience do not differ notable level by gender, computer experience or internet experience. (Contains 7 tables.)