The aim of this study is to investigate the anxiety levels of high school students towards mathematics lesson according to gender variable. A total of 89 ninth grade students (29 females and 60 males) from various high schools were enrolled in Van province center in the 2017-2018 academic year. In the study, the data were collected by using the developed "Mathematical Anxiety Scale" of the researcher. In the analysis of the collected data, frequency and percentage values were taken and independent sample t test was used. According to the results of the study, high school students' mathematics anxiety levels were at medium level. In addition, there were no statistically significant differences in the anxiety levels of female and male students. While the test anxiety levels of the students who participated in the study were higher than the other anxiety levels, the lowest anxiety level was seen as lack of curriculum subjects.