The purpose of the present study was twofold: first to adapt the Relationship and Motivation (REMO) scale addressing role of peers and teachers in students' motivations into Turkish culture, and second to determine whether there were any differences between girls and boys regarding the scores obtained from this scale. To achieve these aims, the present research was designed to be comprised of three consecutive studies. In Study 1, linguistic equivalence was established, and results of an Explanatory Factor Analysis (EFA) performed on data obtained from 202 students showed that structure of the original scale was supported. In Study 2, a Confirmatory Factor Analysis (CFA) was conducted using data obtained from 496 Turkish students, and the results confirmed the results of EFA. Additionally, the validity evidence was obtained by conducting another EFA with 528 students. Moreover, reliability coefficients were also found to be varying in an acceptable range. Including the same participants of Study 2 in Study 3, t-test results showed that girls had significantly higher mean scores on the subscales of peers and teachers as positive motivators, and teachers as negative motivators. On the other hand, boys had significantly higher mean scores on the scale of peers as negative motivators. Results of these studies suggest that Turkish version of REMO is conceptually equivalent to original REMO, and similarly reliable and valid. Therefore, the adapted scale can not only be used in cross-cultural comparison and but also for determining the differentiation in the relations of students with their peers and teachers.