The purpose of this research was to know the effect of blended learning strategy on learning achievement and science process skills of students in plant tissue culture course in the Universitas Negeri Medan. The research method was quasi experiment. The population of this study was all semester VIII students of biology education program. The study sample consists of two classes, namely: class A known as control class who were taught by conventional learning strategy and class C known as experiment class who were taught by blended learning strategy. They were selected using cluster random sampling technique. The results of the study showed that tcount = 3.769, P= 0.001 at the level significance of 0.05 in learning achievements score. It also showed that tcount = 5.435 > ttable = 1.661 P= 0.001 at the level significance of 0.05 in science process skills score. Based on the study result, it can be concluded the blended learning strategy found significantly more effective in enhancing students' learning achievement and science process skills in plant tissue culture course as compared to the conventional learning strategy.