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ERIC Number: EJ990930
Record Type: Journal
Publication Date: 2012
Pages: 4
Abstractor: As Provided
Reference Count: 13
ISSN: ISSN-1470-8175
PCR Analysis of "expR" Gene Regulating Biosynthesis of Exopolysaccharides in "Sinorhizobium Meliloti"
Sorroche, Fernando G.; Giordano, Walter
Biochemistry and Molecular Biology Education, v40 n2 p108-111 Mar-Apr 2012
Exopolysaccharide (EPS) production by the rhizobacterium "Sinorhizobium meliloti" is essential for root nodule formation on its legume host (alfalfa), and for establishment of a nitrogen-fixing symbiosis between the two partners. Production of EPS II (galactoglucan) by certain "S. meliloti" strains results in a mucoid colony phenotype. Other strains that are unable to produce EPS II display a dry phenotype, due to the presence of an insertion element in the gene "expR", a key regulator involved in many important cellular processes, including production of low-molecular-weight EPS II. We describe a series of three programmed undergraduate biochemistry laboratory classes teaching PCR and electrophoresis procedures to detect non-functional "expR" loci in "S. meliloti". (Contains 3 figures and 2 tables.)
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Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: Argentina