Since 1968, biologists have known that termites line up and follow some ballpoint ink lines but not others. Suggestions for class lessons based on this observation have become widespread. However, many of these are incomplete, superficial, conflicting, and/or occasionally inaccurate, and most provide only simple demonstrations or cookbook-style confirmations. Here, we provide added background for this activity to update, clarify, and expand it. Some ways to use termite trail-following to teach fundamental life and physical science concepts through hands-on inquiry are presented, based on our experience with university students and teachers. These activities, adaptable to many instructional levels, range in scope from a single laboratory session to extended investigation.