We describe the development of a mini-library of human DNA fragments for use in an enquiry-based learning (EBL) undergraduate practical incorporating "wet-lab" and bioinformatics tasks. In spite of the widespread emergence of the polymerase chain reaction (PCR), the cloning and analysis of DNA fragments in "Escherichia coli" remains a fundamental skill commonly taught to undergraduate biology students. The individual procedures are simple enough, but enthusiastic engagement of the students in practical exercises requires that a better learning experience be offered to them than the simple opportunity to participate. To address this issue we have prepared a mini-library of well-characterised fragments of human DNA in a small plasmid vector. We have generated high-quality sequence data for each end of the human DNAs and there are sufficient clones for around 80 students to each engage in a series of enquiry-based activities producing results that are unique to each student. This resource allows the students to experience the real-life integration of "wet-lab" and bioinformatics approaches in modern molecular genetics. The experimental procedures are aimed at molecular genetics students commencing their second year of undergraduate study, but the materials could also be used with more advanced groups, up to and including masters degree level, by appropriate adaptation and extension of the range of experimental techniques used. (Contains 4 figures.)