ERIC Number: EJ943960
Record Type: Journal
Publication Date: 2011-Oct
Abstractor: As Provided
Reference Count: 0
Aversive Olfactory Learning and Associative Long-Term Memory in "Caenorhabditis elegans"
Amano, Hisayuki; Maruyama, Ichiro N.
Learning & Memory, v18 n10 p654-665 Oct 2011
The nematode "Caenorhabditis elegans" ("C. elegans") adult hermaphrodite has 302 invariant neurons and is suited for cellular and molecular studies on complex behaviors including learning and memory. Here, we have developed protocols for classical conditioning of worms with 1-propanol, as a conditioned stimulus (CS), and hydrochloride (HCl) (pH 4.0), as an unconditioned stimulus (US). Before the conditioning, worms were attracted to 1-propanol and avoided HCl in chemotaxis assay. In contrast, after massed or spaced training, worms were either not attracted at all to or repelled from 1-propanol on the assay plate. The memory after the spaced training was retained for 24 h, while the memory after the massed training was no longer observable within 3 h. Worms pretreated with transcription and translation inhibitors failed to form the memory by the spaced training, whereas the memory after the massed training was not significantly affected by the inhibitors and was sensitive to cold-shock anesthesia. Therefore, the memories after the spaced and massed trainings can be classified as long-term memory (LTM) and short-term/middle-term memory (STM/MTM), respectively. Consistently, like other organisms including "Aplysia", "Drosophila", and mice, "C. elegans" mutants defective in "nmr-1" encoding an NMDA receptor subunit failed to form both LTM and STM/MTM, while mutations in "crh-1" encoding the CREB transcription factor affected only the LTM.
Descriptors: Stimuli, Classical Conditioning, Long Term Memory, Olfactory Perception, Associative Learning, Cytology, Molecular Structure, Classification, Drug Use, Entomology, Genetics
Cold Spring Harbor Laboratory Press. 500 Sunnyside Boulevard, Woodbury, NY 11797-2924. Tel: 800-843-4388; Tel: 516-367-8800; Fax: 516-422-4097; e-mail: firstname.lastname@example.org; Web site: http://www.learnmem.org/
Publication Type: Journal Articles; Reports - Evaluative
Education Level: N/A
Authoring Institution: N/A