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ERIC Number: EJ943879
Record Type: Journal
Publication Date: 2011-May
Pages: 3
Abstractor: As Provided
Reference Count: 1
ISBN: N/A
ISSN: ISSN-1539-2422
Creating Cost-Effective DNA Size Standards for Use in Teaching and Research Laboratories
Shultz, Jeff
Bioscene: Journal of College Biology Teaching, v37 n1 p23-25 May 2011
I have devised a method with which a molecular size standard can be readily manufactured using Lambda DNA and PCR. This method allows the production of specific sized DNA fragments and is easily performed in a standard molecular biology laboratory. The material required to create these markers can also be used to provide a highly robust and reliable introduction of students to the PCR technique. Protocols for demonstrating the effect of the effect of amplification cycles and DNA and Primer concentration are provided. (Contains 1 table and 1 figure.)
Association of College and Biology Educators. Web site: http://acube.org
Publication Type: Journal Articles; Reports - Descriptive
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A