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ERIC Number: EJ943678
Record Type: Journal
Publication Date: 2011-Sep
Pages: 4
Abstractor: As Provided
Reference Count: 16
ISSN: ISSN-0021-9584
A Comprehensive Enzyme Kinetic Exercise for Biochemistry
Barton, Janice S.
Journal of Chemical Education, v88 n9 p1336-1339 Sep 2011
This article describes a comprehensive treatment of experimental enzyme kinetics strongly coupled to electronic data acquisition and use of spreadsheets to organize data and perform linear and nonlinear least-squares analyses, all in a manner that promotes development of important reasoning skills. Kinetic parameters are obtained for the stable enzyme bovine alkaline phosphatase by fitting the dependence of velocity on substrate and inhibitor concentration using regression algorithms of Excel. (Contains 2 tables and 2 figures.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A