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ERIC Number: EJ941074
Record Type: Journal
Publication Date: 2011-Aug
Pages: 5
Abstractor: As Provided
Reference Count: 14
ISBN: N/A
ISSN: ISSN-0021-9584
Ribose 5-Phosphate Isomerase Investigations for the Undergraduate Biochemistry Laboratory
Jewett, Kathy; Sandwick, Roger K.
Journal of Chemical Education, v88 n8 p1170-1174 Aug 2011
The enzyme ribose 5-phosphate isomerase (RpiA) has many features that make it attractive as a focal point of a semester-long, advanced biochemistry laboratory for undergraduate students. The protein can easily and inexpensively be isolated from spinach using traditional purification techniques. Characterization of RpiA enzyme activity can be assessed using a timed Dische assay or a relatively simple A[subscript 290] kinetic assay. Cloning of the enzyme from "Escherichia coli" works well as a molecular biology exercise to give students experiences in such techniques as polymerase chain reaction, restriction mapping, plasmid purifications, and cell transformations. The use of a common theme throughout the semester more appropriately models the research process and gives the student a better understanding of the procedures included in the pedagogy. (Contains 2 tables and 1 figure.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A