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ERIC Number: EJ940782
Record Type: Journal
Publication Date: 2011-Jun
Pages: 3
Abstractor: As Provided
Reference Count: 14
ISSN: ISSN-0021-9584
Extraction and Purification of Glucoraphanin by Preparative High-Performance Liquid Chromatography (HPLC)
Lee, Iris; Boyce, Mary C.
Journal of Chemical Education, v88 n6 p832-834 Jun 2011
A student activity that focuses on the isolation of glucoraphanin from broccoli using preparative high-performance liquid chromatography (HPLC) is presented here. Glucoraphanin is a glucosinolate, whose byproducts are known to possess anticancer properties. It is present naturally at high levels in broccoli and other "Brassica" vegetables. This activity has students practicing and developing a number of skills including liquid extraction; cleanup by solid-phase extraction; analytical HPLC with diode array detection to identify the peak of interest; and preparative HPLC coupled with a fraction collector to isolate the glucoraphanin fraction. It also represents an authentic application of preparative HPLC as glucoraphanin is not readily available from the main chemical suppliers. (Contains 4 figures.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A