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ERIC Number: EJ939418
Record Type: Journal
Publication Date: 2005
Pages: 4
Abstractor: As Provided
Reference Count: 5
ISBN: N/A
ISSN: ISSN-0021-9266
Colorimetric Quantification and in Situ Detection of Collagen
Esteban, Francisco J.; del Moral, Maria L.; Sanchez-Lopez, Ana M.; Blanco, Santos; Jimenez, Ana; Hernandez, Raquel; Pedrosa, Juan A.; Peinado, Maria A.
Journal of Biological Education, v39 n4 p183-186 2005
A simple multidisciplinary and inexpensive laboratory exercise is proposed, in which the undergraduate student may correlate biochemical and anatomical findings. The entire practical session can be completed in one 2.5-3 hour laboratory period, and consists of the quantification of collagen and total protein content from tissue sections--without previous homogenisation--by a colorimetric assay, and a staining method for in situ collagen detection carried out on the same tissue samples using the same chemicals. Because collagen accumulation is a common characteristic of hepatic diseases such as fibrosis and cirrhosis, the experiment may also serve as the basis for discussing the importance of collagen detection and quantification in estimating the degree of fibrosis from subjects with liver disease. (Contains 2 figures and 4 tables.)
Routledge. Available from: Taylor & Francis, Ltd. 325 Chestnut Street Suite 800, Philadelphia, PA 19106. Tel: 800-354-1420; Fax: 215-625-2940; Web site: http://www.tandf.co.uk/journals
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A