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ERIC Number: EJ938872
Record Type: Journal
Publication Date: 2008-Sep
Pages: 3
Abstractor: As Provided
Reference Count: 8
ISBN: N/A
ISSN: ISSN-1449-6313
Visualising DNA in Classrooms Using Nile Blue
Milne, Christine; Roche, Scott; McKay, David
Teaching Science, v54 n3 p31-33 Sep 2008
Giving students the opportunity to extract, manipulate and visualise DNA molecules enhances a constructivist approach to learning about modern techniques in biology and biotechnology Visualisation usually requires agarose gel electrophoresis and staining. In this article, we report on an alternative DNA stain, Nile Blue A, that may be used in the classroom to visualise DNA. The stain is inexpensive, is at least as sensitive as methylene blue, does not require destaining, requires visualisation with only visible light, and is specific for DNA but not RNA. (Contains 4 figures.)
Australian Science Teachers Association. P.O. Box 334, Deakin West, ACT 2600, Australia. Tel: +61-02-6282-9377; Fax: +61-02-6282-9477; e-mail: publications@asta.edu.au; Web site: http://www.asta.edu.au/resources/teachingscience
Publication Type: Journal Articles; Reports - Research
Education Level: Grade 11; High Schools
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: Australia