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ERIC Number: EJ934688
Record Type: Journal
Publication Date: 2011
Pages: 2
Abstractor: ERIC
Reference Count: 1
ISBN: N/A
ISSN: ISSN-1470-8175
Problem-Based Test: The Effect of Fibroblast Growth Factor on Gene Expression
Szeberenyi, Jozsef
Biochemistry and Molecular Biology Education, v39 n3 p226-227 May-Jun 2011
This paper shows the results of an experiment in which the effects of fibroblast growth factor (FGF), actinomycin D (Act D; an inhibitor of transcription), and cycloheximide (CHX; an inhibitor of translation) were studied on the expression of two genes: a gene called "Fnk" and the gene coding for glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Serum-starved cells were incubated without treatment (sample 1) or treated with FGF for 2 h (samples 2 to 10). After FGF-stimulation, some of the cultures were further treated with actinomycin D (samples 3 to 6) or actinomycin D plus cycloheximide (samples 7 to 10) for various time-periods. Cytoplasmic RNAs were isolated from all cultures and subjected to Northern blot analysis using radioactively labelled Fnk and GAPDH cDNA probes. A problem-based test on the effect of fibroblast growth factor on gene expression is presented. (Contains 1 figure.)
John Wiley & Sons, Inc. Subscription Department, 111 River Street, Hoboken, NJ 07030-5774. Tel: 800-825-7550; Tel: 201-748-6645; Fax: 201-748-6021; e-mail: subinfo@wiley.com; Web site: https://secure.interscience.wiley.com/cgi-bin/jhome/112782101
Publication Type: Journal Articles; Reports - Descriptive; Tests/Questionnaires
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A