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ERIC Number: EJ925600
Record Type: Journal
Publication Date: 2010-Mar
Pages: 4
Abstractor: As Provided
Reference Count: 0
ISBN: N/A
ISSN: ISSN-0002-7685
Making the Most of the "Daphnia" Heart Rate Lab: Optimizing the Use of Ethanol, Nicotine & Caffeine
Corotto, Frank; Ceballos, Darrel; Lee, Adam; Vinson, Lindsey
American Biology Teacher, v72 n3 p176-179 Mar 2010
Students commonly test the effects of chemical agents on the heart rate of the crustacean "Daphnia" magna, but the procedure has never been optimized. We determined the effects of three concentrations of ethanol, nicotine, and caffeine and of a control solution on heart rate in "Daphnia." Ethanol at 5% and 10% (v/v) reduced mean heart rate to appoximately 50% and approximately 20% of its initial value, respectively. Recovery was rapid after removing 5% ethanol, but recovery from 10% ethanol took 20-30 minutes. Nicotine at 100 [mu]M reversibly increased mean heart rate by approximately 20%. Higher concentrations produced varied and sometimes irreversible effects. Caffeine at 0.1%, 0.5%, and 2% (w/v) had no convincing effect on heart rate. Of the three agents tested, nicotine's peculiar effects make it the least useful in an educational setting. Caffeine could be used to emphasize the need for blind observers because it does not increase heart rate in "Daphnia." If students find that it does, their bias is revealed. Ethanol produces unambiguous effects at 5% and 10%. Heart rates recover quickly after removing 5% ethanol, which allows students to explore reversibility as an alternative to having a separate control group.
University of California Press. 2000 Center Street Suite 303, Berkeley, CA 94704. Tel: 510-643-7154; Fax: 510-642-9917; e-mail: customerservice@ucpressjournals.com; Web site: http://www.ucpressjournals.com/journal.asp?j=abt
Publication Type: Journal Articles; Reports - Research
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A