Addressing a drawback in current research on computer-supported collaborative learning (CSCL), this study investigated the influence of motivation on learning activities and knowledge acquisition during CSCL. Participants' (N = 200 university students) task was to develop a handout for which they had first an individual preparing phase followed by a computer-supported collaborative learning phase immediately afterwards. It was hypothesized that in both phases current motivation (in terms of expectancy and value components) influences both learning activities and knowledge acquisition in a positive way. According to main results, only goal orientations (before learning) were associated with knowledge acquisition respectively observed learning activities during the collaborative phase. Expectancy and value components of current motivation related neither to observed learning activities nor to knowledge acquisition during collaborative learning but were in part associated with learning activities and knowledge acquisition during individual learning. The discussion addresses several possible explanations for these unexpected results.