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ERIC Number: EJ923705
Record Type: Journal
Publication Date: 2011-Feb
Pages: 3
Abstractor: As Provided
Reference Count: 13
ISSN: ISSN-0021-9584
An Undergraduate Investigation into the 10-23 DNA Enzyme that Cleaves RNA: DNA Can Cut It in the Biochemistry Laboratory
Flynn-Charlebois, Amber; Burns, Jamie; Chapelliquen, Stephanie; Sanmartino, Holly
Journal of Chemical Education, v88 n2 p226-228 Feb 2011
A low-cost biochemistry experiment is described that demonstrates current techniques in the use of catalytic DNA molecules and introduces a nonradioactive, nonfluorescent, inexpensive, fast, and safe method for monitoring these nucleic acid reactions. The laboratory involves the exploration of the 10-23 DNA enzyme as it cleaves a specific RNA substrate, and the reaction is visualized qualitatively following gel electrophoresis. This experiment provides the students with hands-on experience manipulating nucleic acids using polyacrylamide gel electrophoresis to visually follow the kinetics of the reaction and introduces the student to a novel postelectrophoretic stain, cresyl violet, for detection of the nucleic acids. These DNA enzyme catalysts have potential applications in both biotechnology and medicine and therefore should be of great interest to upper-level undergraduate students. (Contains 4 figures.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A