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ERIC Number: EJ923702
Record Type: Journal
Publication Date: 2011-Feb
Pages: 4
Abstractor: As Provided
Reference Count: 10
ISSN: ISSN-0021-9584
Understanding Fluorescence Measurements through a Guided-Inquiry and Discovery Experiment in Advanced Analytical Laboratory
Wilczek-Vera, Grazyna; Salin, Eric Dunbar
Journal of Chemical Education, v88 n2 p216-219 Feb 2011
An experiment on fluorescence spectroscopy suitable for an advanced analytical laboratory is presented. Its conceptual development used a combination of the expository and discovery styles. The "learn-as-you-go" and direct "hands-on" methodology applied ensures an active role for a student in the process of visualization and discovery of concepts. In this multistep experiment, students start their exploration from the observation of a dark current and the scattered ambient light. Subsequently, they explore properties of the visible light. Signal processing techniques are introduced. After finding the excitation and emission wavelengths for fluorescein, students are able to determine the gross calibration curve. The inner filter effect and spectral shift phenomenon are clearly demonstrated. Using the linear dynamic range of the calibration curve, students then determine the concentration of an unknown. The pH effect on the emission intensity of fluorescence is also studied. (Contains 7 figures.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A