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ERIC Number: EJ921612
Record Type: Journal
Publication Date: 2011-Jan
Pages: 3
Abstractor: As Provided
Reference Count: 8
ISBN: N/A
ISSN: ISSN-0021-9584
Kinetics of Neuraminidase Action on Glycoproteins by One- and Two-Dimensional NMR
Barb, Adam W.; Glushka, John N.; Prestegard, James H.
Journal of Chemical Education, v88 n1 p95-97 Jan 2011
The surfaces of mammalian cells are coated with complex carbohydrates, many terminated with a negatively charged "N"-acetylneuraminic acid residue. This motif is specifically targeted by pathogens, including influenza viruses and many pathogenic bacteria, to gain entry into the cell. A necessary step in the influenza virus life cycle is the release of viral particles from the cell surface; this is achieved by cleaving "N"-acetylneuraminic acid from cell surface glycans with a virally produced neuraminidase. We present a laboratory exercise to model this process using a glycoprotein as a glycan carrier and using real-time nuclear magnetic resonance spectroscopy to monitor "N"-acetylneuraminic acid release as catalyzed by neuraminidase. A time-resolved two-dimensional data-processing technique, statistical total correlation spectroscopy, enhances the resolution of the complicated one-dimensional glycoprotein spectrum and isolates characteristic peaks corresponding to substrates and products. This exercise is relatively straightforward and leads students through a wide range of biologically and chemically relevant procedures, including use of NMR spectroscopy, enzymology, and data-processing techniques. (Contains 5 figures.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A