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ERIC Number: EJ921601
Record Type: Journal
Publication Date: 2011-Jan
Pages: 3
Abstractor: As Provided
Reference Count: 8
ISSN: ISSN-0021-9584
Gradient Scouting in Reversed-Phase HPLC Revisited
Alcazar, A.; Jurado, J. M.; Gonzalez, A. G.
Journal of Chemical Education, v88 n1 p74-76 Jan 2011
Gradient scouting is the best way to decide the most suitable elution mode in reversed-phase high-performance liquid chromatography (RP-HPLC). A simple rule for this decision involves the evaluation of the ratio [delta]t/t[subscript G] (where [delta]t is the difference in the retention time between the last and the first peak and t[subscript G] is the time of the gradient), which is equated to a "magic" reference number. In this article, we demonstrate that the reference number depends on the cosolvent selected. This is illustrated with an example, using methanol as cosolvent. For methanol/water mobile phases, when [delta]t/t[subscript G] less than 0.15, an isocratic elution can be used, otherwise, gradient elution is advisable. In the example, a gradient elution was needed and a segmented gradient program was used to reduce the time of analysis. This article can serve as a reference and basis for laboratory experimentation in an upper-level analytical chemistry course. (Contains 2 tables and 3 figures.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A