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ERIC Number: EJ920489
Record Type: Journal
Publication Date: 2010-Nov
Pages: 4
Abstractor: As Provided
Reference Count: 11
ISSN: ISSN-0021-9584
Introducing Dynamic Combinatorial Chemistry: Probing the Substrate Selectivity of Acetylcholinesterase
Angelin, Marcus; Larsson, Rikard; Vongvilai, Pornrapee; Ramstrom, Olof
Journal of Chemical Education, v87 n11 p1248-1251 Nov 2010
In this laboratory experiment, college students are introduced to dynamic combinatorial chemistry (DCC) and apply it to determine the substrate selectivity of acetylcholinesterase (AChE). Initially, the students construct a chemical library of dynamically interchanging thioesters and thiols. Then, AChE is added and allowed to select and hydrolyze the best substrate, thereby removing it from the equilibrium and subsequently amplifying it. Analysis of these processes is done by [superscript 1]H NMR analysis. The one-day bioorganic laboratory exercise introduces this new and rapidly growing concept in a realistic setting, using a target of biological significance. (Contains 5 figures and 6 notes.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A