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ERIC Number: EJ920344
Record Type: Journal
Publication Date: 2010-Oct
Pages: 3
Abstractor: As Provided
Reference Count: 7
ISBN: N/A
ISSN: ISSN-0021-9584
An Inexpensive, Relatively Green, and Rapid Method to Purify Genomic DNA from "Escherichia Coli": An Experiment for the Undergraduate Biochemistry Laboratory
Sims, Paul A.; Branscum, Katie M.; Kao, Lydia; Keaveny, Virginia R.
Journal of Chemical Education, v87 n10 p1113-1115 Oct 2010
A method to purify genomic DNA from "Escherichia coli" is presented. The method is an amalgam of published methods but has been modified and optimized for use in the undergraduate biochemistry laboratory. Specifically, the method uses Tide Free 2x Ultra laundry detergent, which contains unspecified proteases and lipases, "n"-butanol, 2-propanol, ethanol, and ribonuclease A (or 8 mM sodium hydroxide), and requires a minimum of specialty equipment. Because a number of the required reagents are readily attainable from retail stores, the cost is low enough to allow each student to prepare a sample of purified genomic DNA, even in large laboratory classes. By following the protocol, students are able to purify genomic DNA and use this genomic DNA as a template for a polymerase chain reaction in one 3-h laboratory period. (Contains 3 figures and 3 notes.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A